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Product Details
Synonym
Serum albumin, ALB, Alb
Source
Cynomolgus Serum Albumin, His Tag (CSA-C52H4) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Ala 608 (Accession # A2V9Z4-1).
Predicted N-terminus: Asp 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 67.9 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Cynomolgus Serum Albumin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Cynomolgus Serum Albumin, His Tag (Cat. No. CSA-C52H4) is more than 95% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Cynomolgus Serum Albumin Protein, His Tag (Cat. No. CSA-C52H4) immobilized on CM4 Chip can bind Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-C5284) with an affinity constant of 0.235 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag (SPR verified)(Cat. No. FCM-C82W3) on SA Biosensor, can bind Cynomolgus Serum Albumin, His Tag(Cat. No. CSA-C52H4) with an affinity constant of 0.617 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Serum albumin
- Category：
- Listed Drugs Count：
17 Details
- Clinical Drugs Count：
12 Details
- Highest R&D Stage：
Approved
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