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Materials Provided
IDComponentsSizeRES-A129-C01LER Sample Dilution Buffer15mLApplication
Low Endotoxin Recovery (LER) refers to the phenomenon where the measured endotoxin concentration in a sample is lower than its actual level, which is a common interference issue in both Limulus Amebocyte Lysate (LAL) and recombinant C-factor assays for endotoxin detection; matrix components such as citric acid, ethylenediaminetetraacetic acid (EDTA), Tween 20 and Tween 80 are common triggers of LER, as these substances can either bind to endotoxins, inhibit the enzymatic reaction of LAL reagents or block the activation of recombinant C-factor, resulting in inaccurate and underestimated endotoxin detection results. LER Sample Dilution Buffer can be added to samples containing the aforementioned interfering substances, which effectively mitigates the LER interference and ensures the accuracy of endotoxin quantification in both LAL and recombinant C-factor assays, with an endotoxin content of <0.005 EU/mL that eliminates the risk of introducing exogenous endotoxin contamination during sample preparation.
It is for research use only.
Features
- LER sample dilution buffer: <0.005 EU/mL endotoxin.
- Small pack (15 mL×5 bottles ) to avoid exogenous contamination.
Storage
Store at room temperature.
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Typical Data
Please refer to DS document for the assay protocol.
Endotoxin recovery in 1mM EDTA samples. LER buffer effectively mitigates EDTA-induced endotoxin interference, restoring spiked recovery to 119% (vs. 38% in water).
Endotoxin recovery in 0.05% Tween-80 samples. LER buffer reverses Tween-80-mediated endotoxin masking, achieving 150% spiked recovery (vs. 35% in water).
Endotoxin recovery in 0.01% Tween-20 samples. LER buffer eliminates Tween-20-induced endotoxin interference, improving spiked recovery to 137% (vs. 38% in water).
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