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Product Details
Synonym
RPG, Protein G
Source
Recombinant Protein G, His Tag, also known as Recombinant Streptococcus sp. Protein G was expressed in E. coli at ACRObiosystems.
Molecular Characterization
Recombinant Protein G, His Tag is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 29 kDa polypeptide. Protein G Protein can bind to human IgG1 Fc.
Purity
>98% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 100 mM NaH2PO4, pH8.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Recombinant Protein G, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 98%.
Bioactivity-ELISA
Immobilized Recombinant Protein G, His Tag (Cat. No. RPG-S3140) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag (Cat. No. IG1-H8213) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human IgG1 Fc, Avitag (Cat. No. IG1-H8213) on SA Biosensor, can bind Recombinant Protein G, His Tag (Cat. No. RPG-S3140) with an affinity constant of 0.747 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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