Nipah virus Post-Fusion glycoprotein, His Tag (MALS verified)

Synonym

Postfusion glycoprotein F0/post-F protein (NiV)

Source

Nipah virus Post-Fusion glycoprotein, His Tag (PON-V52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Asn 99 & Gly 117 - Ser 488 (Accession # Q9IH63).

Predicted N-terminus: Ile 27

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 54.5 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Within the expressed region, several mutations were introduced to optimize the protein expression and stability.

The protein is designed as a trimer.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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