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Human gp130 / CD130 / IL-6 R beta Protein, His Tag (MALS verified)

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Product Number/Spec
Price
Menge
ILT-H52H2-100ug
$345.00
ILT-H52H2-1mg (500ug X 2)
$2225.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonym

    IL6ST, gp130, CD130, IL-6RB, IL-6R-beta, CDw130

  • Source

    Human gp130, His Tag (ILT-H52H2) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Glu 619 (Accession # P40189-1).

    Predicted N-terminus: Glu 23

    Request for sequence
  • Molecular Characterization

    IL-6 R beta Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 69.7 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    IL-6 R beta SDS-PAGE

    Human gp130, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    IL-6 R beta SEC-MALS

    The purity of Human gp130, His Tag (Cat. No. ILT-H52H2) is more than 90% and the molecular weight of this protein is around 85-105 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-6 R beta ELISA

    Immobilized Biotinylated Human IL-6 R alpha, Fc,Avitag (Cat. No. ILR-H82F9) at 10 μg/mL (100 μL/well) on Human IL-6, premium grade (Cat. No. IL6-H4218) precoated (0.5 μg/well) plate can bind Human gp130, His Tag (Cat. No. ILT-H52H2) with a linear range of 0.005-0.313 μg/mL (QC tested).

    Protocol
  •  IL-6 R beta ELISA

    Immobilized Human gp130, His Tag (Cat. No. ILT-H52H2) at 5 μg/mL (100 μL/well) can bind Human IL-6, premium grade (Cat. No. IL6-H4218) in the presence of Biotinylated Human IL-6 R alpha, Fc,Avitag (Cat. No. ILR-H82F9) with a linear range of 0.6-20 ng/mL (Routinely tested).

    Protocol

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Publish Comment

Background Introduction

Interleukin-6 receptor subunit beta (IL6ST) is also known as IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, Interleukin-6 signal transducer, Membrane glycoprotein 130 (gp130), CD130, Oncostatin-M receptor subunit alpha and Il6st,which is single-pass type I membrane protein. IL6ST /gp130 /CD130 can be found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver and found in all the cell lines tested except BaF-B03.The expression of IL-6ST /gp130 is not restricted to IL6-responsive cells. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. IL6ST /CD130 can bind to IL6 /IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduce the signal. IL6ST /GP130 does not bind IL6 and may have a role in embryonic development.

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