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Product Details
Synonym
IGHG4
Source
Biotinylated Human IgG4 Fc Protein, Avitag (IG4-H8213) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 327 (Accession # P01861-1).
Predicted N-terminus: Glu 99
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus.
The protein has a calculated MW of 28.0 kDa. The protein migrates as 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human IgG4 Fc Protein, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IgG4 Fc Protein, Avitag (Cat. No. IG4-H8213) is more than 95% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Protein A at 1 μg/mL (100 μL/well) can bind Biotinylated Human IgG4 Fc Protein, Avitag (Cat. No. IG4-H8213) with a linear range of 0.08-1 ng/mL (QC tested).
Protocol
Immobilized Human CD64, His Tag (Cat. No. FCA-H52H1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IgG4 Fc Protein, Avitag (Cat. No. IG4-H8213) with a linear range of 0.08-39 ng/mL (Routinely tested).
Protocol
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