Collect Product
Product Details
Synonym
Ig gamma-3 chain C region, IgG, IgG3
Source
Biotinylated Human IgG3 Fc Protein, Avitag (IG3-H8213) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 377 (Accession # P01860-1).
Predicted N-terminus: Gly
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the N-terminus.
The protein has a calculated MW of 33.4 kDa. The protein migrates as 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Kunden haben auch angesehen
Data Display
SDS-PAGE
Biotinylated Human IgG3 Fc Protein, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IgG3 Fc Protein, Avitag (Cat. No. IG3-H8213) is more than 95% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD64, His Tag (Cat. No. FCA-H52H1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IgG3 Fc Protein, Avitag (Cat. No. IG3-H8213) with a linear range of 0.08-2 ng/mL (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Immunoglobulin G
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
7 Details
- Highest R&D Stage：
Approved
Contact Us
Drug Candidate Licensing
