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Product Details
Synonym
Ig gamma-3 chain C region, IgG, IgG3
Source
Human IgG3 Fc, Tag Free (IG3-H5200) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 377 (Accession # P01860).
Predicted N-terminus: Glu 99
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 31.2 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human IgG3 Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) is more than 95% and the molecular weight of this protein is around 58-71 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) with an affinity constant of 2.82 μM as determined in SPR assay (Biacore 8K) (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Immunoglobulin G
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
7 Details
- Highest R&D Stage：
Approved
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