Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F) Protein, His Tag-Cy5

Synonym

IgG1

Source

Human IgG1 Fc Protein, His Tag-Cy5 (IG1-HC2H3) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1 (C103S, M135Y, S137T, T139E, H316K, N317F)).

Predicted N-terminus: Glu 99

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 28.1 kDa.

Conjugate

Cy5

Excitation Wavelength: 651 nm

Emission Wavelength: 670 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with Cy5 using standard chemical labeling method. The residual Cy5 is removed by molecular sieve treatment during purification process.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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