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Product Details
Synonym
IgG1
Source
Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F), His Tag (IG1-H52H8) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1 (C103S, M135Y, S137T, T139E, H316K, N317F)).
Predicted N-terminus: Glu 99
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.1 kDa. The protein migrates as 33-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F), His Tag (Cat. No. IG1-H52H8) is more than 95% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F), His Tag (Cat. No. IG1-H52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.4-13 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F), His Tag (Cat. No. IG1-H52H8) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 8.45 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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