Mouse GPRC5D Protein, Flag,His Tag (Detergent)

Synonym

GPRC5D, G-protein coupled receptor family C group 5 member D

Source

Mouse GPRC5D Protein, Flag,His Tag (Detergent) (GPD-M52D3) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Cys 300 (Accession # Q9JIL6-2).

Predicted N-terminus: Asp

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a flag tag at the N-terminus and a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 38.3 kDa. The protein migrates as 33-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>80% as determined by SDS-PAGE.

Formulation

This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping

This product is supplied and shipped with dry ice, please inquire the shipping cost.

Storage

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 3 months under sterile conditions.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process