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Product Details
Synonym
GM-CSF R alpha, GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, CSF2RA, CSF2R, CSF2RY, CDw116, CD116
Source
Human GM-CSF R alpha, His Tag (GRA-H52H7) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1).
Predicted N-terminus: Glu 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.4 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human GM-CSF R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Biotinylated Human GM-CSF, epitope tag free, primary amine labeling (Cat. No. GMF-H8214) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.2 μg/well) plate can bind Human GM-CSF R alpha, His Tag (Cat. No. GRA-H52H7) with a linear range of 0.039-0.313 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human GM-CSF R alpha, His Tag (Cat. No. GRA-H52H7) immobilized on CM5 Chip can bind Human GM-CSF, premium grade (Cat. No. GMF-H4214) with an affinity constant of 65.2 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human GM-CSF, epitope tag free, primary amine labeling (Cat. No. GMF-H8214) on SA Biosensor, can bind Human GM-CSF R alpha, His Tag (Cat. No. GRA-H52H7) with an affinity constant of 23.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Phase 3 Clinical
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