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Product Details
Synonym
EGF, URG, HOMG4
Source
Human EGF Protein, His Tag (EGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Sterility
Negative
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human EGF Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-Organoid Culture
EGF (Cat. No. EGF-H52H3) maintains intestinal organoid growth well through multiple passages and long-term culture, comparable to competing companies. The organoids showed good budding morphology.
Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 (Cat. No. RS6-H4220), FGF7 (Cat. No. FG7-H52H5), FGF10, HGF (Cat. No. HGF-H52H3) actively support liver ductal organoid growth.
iPSC derived intestinal organoids forming cryptic structure were cultured with Human EGF (Cat. No. EGF-H52H3).
Bioactivity-ELISA
Immobilized Human EGF Protein, His Tag (Cat. No. EGF-H52H3) at 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.2-5 ng/mL (QC tested).
Protocol
Bioactivity-CELL BASE
Human EGF Protein, His Tag (Cat. No. EGF-H52H3) stimulates EGFR (Luc) HEK293 Reporter Cell. The specific activity of Human EGF Protein, His Tag is > 3.00 X 10^5 IU/mg, which is calibrated against human growth factor EGF WHO International Standard (NIBSC code: 91/530) (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Epidermal growth factor
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Phase 2 Clinical
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