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Product Details
Synonym
EGF, URG, HOMG4
Source
Human EGF, Mouse IgG2a Fc Tag (EGF-H525b) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).
Predicted N-terminus: Glu
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the N-terminus.
The protein has a calculated MW of 33.1 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human EGF, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human EGF R, Fc Tag (Cat. No. EGR-H5252) at 5 μg/mL (100 μL/well)can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with a linear range of 0.3-10 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human EGF R, His Tag (Cat. No. EGR-H5222) captured on CM5 Chip via anti-His antibody can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with an affinity constant of 2.63 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Human EGF R Protein, His Tag (Cat. No. EGR-H522a) captured on CM5 Chip via anti-His antibody can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with an affinity constant of 2.76 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Epidermal growth factor
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Phase 2 Clinical
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