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Product Details
Synonym
Delta3, delta-like 3 (Drosophila), delta-like protein 3, DLL3, Pudgy, SCDO1, SCDO1delta3
Source
Cynomolgus DLL3, His Tag (DL3-C52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 490 (Accession # XP_005589253.1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 50.4 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus DLL3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus DLL3, His Tag (Cat. No. DL3-C52H3) is more than 95% and the molecular weight of this protein is around 75-100 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus DLL3, His Tag (Cat. No. DL3-C52H3) at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding DSL of DLL3), Human IgG1 with a linear range of 0.1-0.8 ng/mL (QC tested).
Protocol
Bridging ELISA
Validated with Bispecific Antibody.
Immobilized Cynomolgus DLL3 Protein, His Tag (Cat. No. DL3-C52H3) at 1 μg/mL, add increasing concentrations of Tarlatamab and then add Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 3.91 ng/mL (Routinely tested).
Bioactivity-SPR
Bispecific T-cell Engager captured on Protein A Chip can bind Cynomolgus DLL3, His Tag (Cat. No. DL3-C52H3) with an affinity constant of 0.792 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Delta-like protein 3
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
28 Details
- Highest Development Stage：
Approved
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