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Product Details
Synonym
Claudin-6, CLDN6
Source
Human Claudin-6 Protein, His,Twin-Strep Tag (CL6-H5289) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Val 220 (Accession # P56747-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus and a twin strep tag at the C-terminus.
The protein has a calculated MW of 28.3 kDa. The protein migrates as 26-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Purity
>80% as determined by SDS-PAGE.
Formulation
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
ACRO Quality Management System
- *The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
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Data Display
SDS-PAGE
Human Claudin-6 Protein, His,Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human Claudin-6 Protein, His,Twin-Strep Tag (Cat. No. CL6-H5289) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin-6 antibody, Human IgG1 | Human Kappa with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-Claudin-6 Antibody, Human IgG1 captured on Protein A Chip can bind Human Claudin-6 Protein, His,Twin-Strep Tag (Cat. No. CL6-H5289) with an affinity constant of 4.95 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Claudin 6
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
15 Details
- Highest R&D Stage：
Phase 2 Clinical
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Drug Candidate Licensing
