Biotinylated Human Cadherin-17 / CDH17 Protein, His,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
CA7-H52E6-25ug
$505.00
CA7-H52E6-200ug
$1845.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    Cadherin-17, CDH17, HPT-1, LI-cadherin

  • Source

    Biotinylated Human Cadherin-17, His,Avitag (CA7-H52E6) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Met 787 (Accession # Q12864-1).

    Predicted N-terminus: Gln 23

    Request for sequence
  • Molecular Characterization

    Cadherin-17 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 88.6 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Cadherin-17 SDS-PAGE

    Biotinylated Human Cadherin-17, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Cadherin-17 SEC-MALS

    The purity of Biotinylated Human Cadherin-17, His,Avitag (Cat. No. CA7-H52E6) is more than 90% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Cadherin-17 ELISA

    Immobilized Human Anti-Cadherin 17 at 0.1% can bind Biotinylated Human Cadherin-17, His,Avitag (Cat. No. CA7-H52E6) with a linear range of 0.2-16 ng/mL (QC tested).

    Protocol
  •  Cadherin-17 ELISA

    Immobilized Biotinylated Human Cadherin-17, His,Avitag (Cat. No. CA7-H52E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind various dilution ratio of Human Anti-Cadherin 17 (Routinely tested).

    Protocol

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Background

Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.

Recent Advances

 
Drug Development Progress
  • English Name:

    Cadherin-17

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    17 Details

  • Highest Development Stage:

    Phase 2 Clinical

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  • Background