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Product Details
Product Details
This antibody panel comprises three distinct rabbit monoclonal antibodies against Eribulin, with slight differences in their recognition epitopes. Consequently, they may exhibit varying affinity levels towards different Eribulin analogs or different conjugates of Eribulin and linkers. This panel can be used to conduct preliminary experiments for screening the optimal antibody clone.
Components
Cat.No.CloneSizeERN-MY2062b1M1F520 ugERN-MY2063b1M2B1120 ugERN-MY2012b1M1G1120 ugSpecificity
Specifically recognizes Eribulin.
Source
This panel contains three anti-Eribulin monoclonal antibodies, suitable for pharmacokinetic (PK) detection of ADC with Eribulin as the payload. All the anti-Eribulin monoclonal antibodies are recombinantly expressed from HEK293, can be used as a capture antibody for detecting conjugated antibodies in ELISA assay.
Isotype
Rabbit IgG | Rabbit Kappa
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
Bioactivity-ELISA
Immobilized Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1F5) (Cat. No. ERN-MY2062b) at 1 μg/mL, add Farletuzumab Ecteribulin in the 100% Human Serum and then add Biotinylated Human FOLR1, Fc,Avitag (Cat. No. FO1-H82F9) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M2B11) (Cat. No. ERN-MY2063b) at 1 μg/mL, add Farletuzumab Ecteribulin in the 100% Human Serum and then add Biotinylated Human FOLR1, Fc,Avitag (Cat. No. FO1-H82F9) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1G11) (Cat. No. ERN-MY2012b) at 1 μg/mL, add Farletuzumab Ecteribulin in the 100% Human Serum and then add Biotinylated Human FOLR1, His,Avitag (Cat. No. FO1-H82E2) at 1.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
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