Product Details
Materials Provided
IDComponentsSizeBIS009-C01Pre-coated Human VEGFA Protein Microplate1 plate (8×12 strips)BIS009-C02Anti-PD-L1 & VEGF Antibody Standard20 μgBIS009-C03Biotin-Human PD-L1 Protein20 μgBIS009-C04Streptavidin-HRP20 μgBIS009-C0510×Washing Buffer50 mLBIS009-C062×Dilution Buffer50 mLBIS009-C07Substrate Solution12 mLBIS009-C08Stop Solution7 mLProduct Overview
The Bispecific PD-L1:VEGFA Bridging ELISA Kit is a comprehensive, ready-to-use assay designed to functionally confirm the simultaneous binding of anti-PD-L1-anti-VEGFA bispecific antibodies (BsAbs) to both of their intended targets.This assay quantifies the formation of the critical PD-L1-BsAb-VEGFA trimolecular complex, providing a direct measure of the antibody's primary mechanism of action. This all-inclusive kit contains all essential reagents for 100 reactions, including recombinant human VEGFA, a PD-L1-containing detection reagent, and a validated positive control antibody, thereby streamlining the screening and profiling of therapeutic candidates with exceptional precision and reliability.
Storage
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.

Detection of anti-PD-L1 & VEGF antibodies by Bridging ELISA Assay.
Bispecific Human PD-L1 & VEGFA Bridging ELISA Kit can specifically identify Anti-PD-L1 & VEGF Antibodies, with linear range of 0.006-0.181 nM (QC tested).
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