Product Details
Materials Provided
IDComponentsSizeBIS004-C01Pre-coated Human Claudin-18.2 Protein Microplate1 plate (8×12 strips)BIS004-C02Anti-CD3 & Claudin-18.2 Antibody Standard20 μgBIS004-C03Biotin-Human CD3E Protein20 μgBIS004-C04Streptavidin-HRP20 μgBIS004-C0510×Washing Buffer50 mLBIS004-C062×Dilution Buffer50 mLBIS004-C07Substrate Solution12 mLBIS004-C08Stop Solution7 mLProduct Overview
The Bispecific Claudin-18.2:CD3 Bridging ELISA Kit is a comprehensive, ready-to-use assay designed to functionally confirm the simultaneous binding of anti-Claudin-18.2-anti-CD3 bispecific antibodies (BsAbs) to both of their intended targets. This assay quantifies the formation of the critical Claudin-18.2-BsAb-CD3 trimolecular complex, providing a direct measure of the antibody's primary mechanism of action. This all-inclusive kit contains all essential reagents for 100 reactions, including recombinant human Claudin-18.2, a CD3-containing detection reagent, and a validated positive control antibody, thereby streamlining the screening and profiling of therapeutic candidates with exceptional precision and reliability.
Storage
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.
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Data Display
Typical Data
Please refer to DS document for the assay protocol.

Detection of anti-CD3 & Claudin-18.2 antibodies by Bridging ELISA Assay.
Bispecific Human Claudin-18.2 & CD3E Bridging ELISA Kit can specifically identify Anti-CD3 & Claudin-18.2 Antibodies, with linear range of 0.184-5.874 nM (QC tested).
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