Bispecific Human CD19 & CD3 Bridging ELISA Kit

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Product Details

  • Materials Provided

    ID
    Components
    Size
    BIS002-C01
    Pre-coated Human CD3E & CD3G Protein Microplate
    1 plate (8×12 strips)
    BIS002-C02
    Anti-CD3 & CD19 Antibody Standard
    20 μg
    BIS002-C03
    Biotin-Human CD19 Protein
    20 μg
    BIS002-C04
    Streptavidin-HRP
    20 μg
    BIS002-C05
    10×Washing Buffer
    50 mL
    BIS002-C06
    2×Dilution Buffer
    50 mL
    BIS002-C07
    Substrate Solution
    12 mL
    BIS002-C08
    Stop Solution
    7 mL

  • Product Overview

    The Bispecific CD19:CD3 Bridging ELISA Kit is a comprehensive, ready-to-use assay designed to functionally confirm the simultaneous binding of anti-CD19-anti-CD3 bispecific antibodies (BsAbs) to both of their intended targets.This assay quantifies the formation of the critical CD19-BsAb-CD3 trimolecular complex, providing a direct measure of the antibody's primary mechanism of action. This all-inclusive kit contains all essential reagents for 100 reactions, including recombinant human CD19, a CD3-containing detection reagent, and a validated positive control antibody, thereby streamlining the screening and profiling of therapeutic candidates with exceptional precision and reliability.

  • Storage

    1. Store the unopened kit at 2-8°C upon receipt.

    2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.

    3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Typical Data 

    Please refer to DS document for the assay protocol.

     CD19 & CD3E TYPICAL DATA

    Detection of anti-CD3 & CD19 antibodies by Bridging ELISA Assay.
    Bispecific Human CD19 & CD3 Bridging ELISA Kit can specifically identify Anti-CD3 & CD19 Antibodies, with linear range of 0.090-5.787 nM (QC tested).

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