ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade

DMF

This product can be directly isolated using conventional column-free magnetic stand (eg. Cat. MB-01/ MB-02/ MB-03/ MB-04). Please note that due to its micron-scale size, it is not compatible with commercially available column-based (M/D/L) magnetic separation systems.

Fig.1. Activation of the purified human T Cells.The human T cells were stimulated with ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade (Cat. No. MBS-C001) for 24hrs, and the activation was assessed by measuring expression of both activation markers CD25 and CD69 expression on the T cells surface by staining with PE labeled anti-human CD25 antibody and FITC labeled anti-human CD69 antibody respectively (QC tested).

Fig.2. Purified human T Cells expansion.The human T cells were labeled with carboxy fluorescein succinimidyl ester (CFSE) and stimulated with ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade(Cat. No. MBS-C001), and then the proliferation of the T cells was assessed with CFSE dilution assay by flow cytometry on day 5 after stimulation (QC tested).

Activation of the purified human T Cells. The purified human T cells were activated using Human T cell Activation/Expansion CD3/CD28 Beads, (ACRO, Cat. No. GMP-MBS001/MBS-C001) respectively for 24 hours with CTS Optimizer Medium. Cells were fluorescently stained using PE labeled anti-human CD25 antibody and labeled FITC anti-human CD69 antibody and analyzed by flow cytometry.

Expansion of the human CD3+T cells. Human T cells using ACROBiosystems CD3/CD28 Beads (ACRO, Cat. No. GMP-MBS001/MBS-C001) were expanded under two different medium, respectively. Expansion was performed for two weeks, showing that ACROBiosystems’ GMP and PG beads showing similar proliferative abilities.

PD-1 expression of the activated human T Cells. The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads at a ratio of 1:1 beads-to-cells. Cells were expanded in T cell culture medium supplemented with 4ng/mL of rhIL-2 Protein. Activated T cells were expanded for up to 8 days with low PD-1 expression.