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Product Details
- ItemsDetailsDetection MethodChemiluminescenceProduct TypeMagnetic Beads (Anti-His Tag Antibody, Human IgG1)Quantity Size10mg / 100mgPhysical Appearancelyophilized powder mixtureParticle size2.8µmBeads SurfaceHydrophilicBinding Capacity3-8μg His tag proteins / mg beadsEmission WavelengthMeasured relative light units (RLU) at 430 nm
Product Description
The Anti-His Tag-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal Anti-His Tag antibody. The beads can be used to capture the proteins containing C-terminal, N-terminal, and internal His tagged fusion in Chemiluminescence procedures. The Monoclonal Anti-His Tag antibody is a chimeric monoclonal antibody expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The Anti-His Tag-coupled Magnetic Beads is easy to capture his Tagged protein or other molecules, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
Application
The Anti-His Tag-coupled Magnetic Beads is used to capture his Tagged protein or molecules, it can combination with Acridinium ester markers in chemiluminescence technology. The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, AMG-Acridinium ester can capture mouse antibodies, this allows detection of ligand / acceptor binding assay, antigen/antibody binding assay, or antibody screening.
Reconstitution
See Certificate of Analysis (CoA) for detailed instruction.
Storage
Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
Formulation
Please contact us for detailed information.
Contact us for customized product form or formulation.
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Bioactivity-MPCLIA
Immobilized 0.025 μg /Test of SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) to the Anti-His Tag Antibody (Human IgG1)-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A007) (10 μg beads/Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Mouse IgG1 (AM122) (Cat. No. SCT-M369) at increasing concentration coupled to Anti-Mouse IgG-Acridine ester (Cat. No. AMG-S163, 0.04 μg /Test). Detection was performed with sensitivity of 0.98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Anti-His Tag Antibody (Human IgG1)-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A007) is stable at 37°C for 10 days.
The MPCLIA assay shows that Anti-His Tag Antibody (Human IgG1)-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A007) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Anti-His Tag Antibody (Human IgG1)-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A007) is stable at 37°C for 10 days.
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