Product Details
Source
Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
Clone
6F2F7-2
Species
Mouse
Isotype
Mouse IgG1 | Mouse Kappa
Antibody Type
Recombinant Monoclonal
Immunogen
Trastuzumab
Specificity
Recognizes Trastuzumab specifically.
Application
ApplicationRecommended UsageELISA0.2-25 ng/mLPurity
95% as determined by SDS-PAGE.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from a 0.22 μm-filtered solution in pH 7.5 containing Tris with Glycine, Arginine and NaCl, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE

Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA

Immobilized Trastuzumab at 1 μg/mL (100 μL/well) can bind Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) (Cat. No. TRB-Y5b) with a linear range of 0.19-6.25 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) ( Cat. No. TRB-Y5b) was detected by Biotinylated Human Her2, His,Avitag (Cat. No. HE2-H82E2) with a half maximal inhibitory concentration (IC50) of 7.02 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR

Trastuzumab captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) (Cat. No. TRB-Y5b) with an affinity constant of 0.915 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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