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Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) (recommended for PK/PD)

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Product Number/Spec
Price
Menge
TRB-Y5b-100ug
$355.00
TRB-Y5b-1mg (250ug X 4)
$2695.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Source

    Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • Clone

    6F2F7-2

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Antibody Type

    Recombinant Monoclonal

  • Immunogen

    Trastuzumab

  • Specificity

    Recognizes Trastuzumab specifically.

  • Application

    Application
    Recommended Usage
    ELISA
    0.2-25 ng/mL
  • Purity

    95% as determined by SDS-PAGE.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in pH 7.5 containing Tris with Glycine, Arginine and NaCl, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    Trastuzumab SDS-PAGE

    Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Trastuzumab ELISA

    Immobilized Trastuzumab at 1 μg/mL (100 μL/well) can bind Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) (Cat. No. TRB-Y5b) with a linear range of 0.19-6.25 ng/mL (QC tested).

    Protocol
  •  Trastuzumab ELISA

    Serial dilutions of Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) ( Cat. No. TRB-Y5b) was detected by Biotinylated Human Her2, His,Avitag (Cat. No. HE2-H82E2) with a half maximal inhibitory concentration (IC50) of 7.02 μg/mL (Routinely tested).

    Protocol
  • Bioactivity-SPR

     Trastuzumab SPR

    Trastuzumab captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) (Cat. No. TRB-Y5b) with an affinity constant of 0.915 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Publish Comment

Background Introduction

Trastuzumab, is a monoclonal antibody used to treat breast cancer. Specifically it is used for breast cancer that is HER2 receptor positive. Trastuzumab works by binding to the HER2 receptor and slowing down cell duplication. Trastuzumab is a recombinant IgG1 kappa, humanized monoclonal antibody that selectively binds with high affinity in a cell-based assay (Kd = 5 nM) to the extracellular domain of the human epidermal growth factor receptor protein.

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