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Product Details
Source
Anti-Trastuzumab Antibody (AY1b) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
Clone
AY1b
Species
Mouse
Isotype
Mouse IgG1/kappa
Antibody Type
Recombinant Monoclonal
Immunogen
Trastuzumab
Specificity
Recognizes Trastuzumab specifically.
Application
ApplicationRecommended UsageELISA0.39-100 ng/mLPurity
95% as determined by SDS-PAGE.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from a 0.22 μm-filtered solution in pH 7.5 containing Tris with Glycine, Arginine and NaCl, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Anti-Trastuzumab Antibody (AY1b) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Trastuzumab at 1 μg/mL (100 μL/well) can bind Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) at 2 μg/mL, add increasing concentrations of Trastuzumab in the 10% human serum and then add Biotinylated Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) (Cat. No. TRA-BLY5b) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 48 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) was detected by Biotinylated Human Her2, His,Avitag (Cat. No. HE2-H82E2) with a half maximal inhibitory concentration (IC50) of 4.58 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Trastuzumab captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) with an affinity constant of 0.729 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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