Product Details
Source
Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
Clone
4F12
Species
Mouse
Isotype
Human IgG1 | Human Kappa
Conjugate
Unconjugated
Antibody Type
Recombinant Monoclonal
Reactivity
Varicella zoster virus (strain Oka vaccine)
Immunogen
Recombinant Varicella zoster virus (strain Oka vaccine) gH&gL Protein is expressed from human 293 cells
Specificity
Specifically recognizes Varicella zoster virus (strain Oka vaccine) Protein.
Application
ApplicationRecommended UsageELISA0.1-63 ng/mLPurity
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE

Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) (Cat. No. GHL-MY2111) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Varicella zoster virus (strain Oka vaccine) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-V5283) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) (Cat. No. GHL-MY2111) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
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