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Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) (MALS verified)

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Product Number/Spec
Price
Menge
GHL-MY2111-100ug
$400.00
GHL-MY2111-1mg
$2655.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Source

    Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    4F12

  • Species

    Mouse

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Varicella zoster virus (strain Oka vaccine)

  • Immunogen

    Recombinant Varicella zoster virus (strain Oka vaccine) gH&gL Protein is expressed from human 293 cells

  • Specificity

    Specifically recognizes Varicella zoster virus (strain Oka vaccine) Protein.

  • Application

    Application
    Recommended Usage
    ELISA
    0.1-63 ng/mL
  • Purity

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    gH&gL SDS-PAGE

    Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    gH&gL SEC-MALS

    The purity of Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) (Cat. No. GHL-MY2111) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     gH&gL ELISA

    Immobilized Varicella zoster virus (strain Oka vaccine) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-V5283) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) (Cat. No. GHL-MY2111) with a linear range of 0.1-4 ng/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

Varicella-zoster virus (VZV) is the alphaherpesvirus that causes chicken pox (varicella) and shingles (zoster). As for all herpesviruses, VZV relies on a fusion complex comprised of three core glycoproteins, gB, gH and gL, required for entry of virions into host cells. VZV ORFs 37 and 60 encode gH (841aa; 118 kDa) and gL (160aa; 20 kDa), respectively, which are N- and O-linked glycosylated and form a heterodimer that is necessary for the activation of gB.

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