Product Details
Source
Rabbit Anti-Mouse FMC63 Polyclonal Antibody is a polyclonal antibody purified from rabbit serum.
Species
Rabbit
Conjugate
Unconjugated
Antibody Type
Polyclonal Antibody
Reactivity
Mouse
Immunogen
FMC63 scFv
Specificity
Recognizes FMC63 scFv specifically.
Application
ApplicationRecommended UsageELISA0.1-10 ng/mLPurity
90% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE

Rabbit Anti-Mouse FMC63 Polyclonal Antibody on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS

The purity of Rabbit Anti-Mouse FMC63 Polyclonal Antibody (Cat. No. FM3-S93) is more than 95% and the molecular weight of this protein is around 130-145 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Mouse FMC63 Polyclonal Antibody (Cat. No. FM3-S93) with a linear range of 0.098-0.78 ng/mL (QC tested).
Protocol
Immobilized anti-CD19 antibody (Clone: FMC63) at 1 μg/mL, add increasing concentrations of Rabbit Anti-Mouse FMC63 Polyclonal Antibody (Cat. No. FM3-S93) and then add Biotinylated anti-CD19 antibody (Clone: FMC63) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 78 ng/mL (QC tested).
Protocol
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