APC-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) (0.03% Proclin)

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Cat. No. / Size
Price
Qty
FM3-AFM721-25tests
$316.00
FM3-AFM721-200tests
$1960.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) is a neutralizing rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.

  • Application

    Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).

  • Clone

    1G10

  • Species

    Rabbit

  • Isotype

    Rabbit IgG | Rabbit Kappa

  • Specificity

    Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.

  • Immunogen

    Recombinant FMC63 scFv derived from HEK293 cells

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Recommended Dilution

    1:50

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8 °C for 12 months after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     FMC63 FACS

    Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with APC-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) (Cat. No. FM3-AFM721) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).

    Protocol

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Background

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.

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