Product Details
Source
FMC63 scFv, His Tag is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
Species
Mouse
Isotype
C-10×His
Conjugate
Unconjugated
Antibody Type
scFV
Reactivity
Human
Immunogen
CD19
Specificity
Specifically recognizes the CD19.
Application
ApplicationRecommended UsageELISA5-125 ng/mLPurity
95% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE

FMC63 scFv, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) is more than 95% and the molecular weight of this protein is around 25-37 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) at 5 μg/mL (100 μL/well) can bind FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
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